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Promega mouse anti-b-gal
Mouse Anti B Gal, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+anti-b-gal/mouse+anti+%CE%B2+galactosidase/pm39513270-251-26-28
Average 90 stars, based on 1 article reviews
mouse anti-b-gal - by Bioz Stars, 2026-11
90/100 stars

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Related Articles

Recombinant:

Article Title: Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila.
Article Snippet: Melanotic nodules are commonly assumed to be caused by an abnormal immune response.. Several hematopoietic mutants and signaling pathways, including the Toll, JAK/STAT, Ras and JNK pathways, can cause melanotic nodules to develop when specifically activated in hemocytes.. Here, we used the UASGal4 system to overexpress jumeaux (jumu) in the fly immune response system.

Article Title: Inverse regulation of target genes at the brink of the BMP morphogen activity gradient.
Article Snippet: BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates.. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk).. This patterning model is inadequate to explain the expression of target genes, like vestigial and spalt, in lateral regions of the wing disc where BMP signals decline and Brk levels peak.

Article Title: APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Article Snippet: Mouse anti-B-gal , Promega , RRID: AB_2314509.

Article Title: Notch Mediates Inter-tissue Communication to Promote Tumorigenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-GFP Abcam Cat# ab6673; RRID: AB_305643 Mouse anti-Cut DSHB Cat# 2b10; RRID: AB_528186 Rat anti-DE-Cad2 DSHB Cat# DCAD2; RRID: AB_528120 Mouse anti-Delta DHSB Cat# c594.9b; RRID: AB_528194 Mouse anti-b-Gal Promega Cat# Z378A; RRID: AB_2313752 Rat anti-Tropomyosin Abcam Cat# ab50567; RRID: AB_883223 Rabbit anti-Zfh1 Ruth Lehmann N/A Rabbit anti-Mef2 Eileen Furlong N/A Rabbit anti-Twist Eileen Furlong N/A Mouse anti-pH3 Cell Signaling Technology Cat# 9706; RRID: AB_331748 Mouse Anti-NICD DSHB Cat# c17.9c6; RRID: AB_528410 Chemicals, Peptides, and Recombinant Proteins TRIzol ThermoFisher Scientific Cat# 15596026 Oligo(dT)15 primers Promega Cat# C1101 M-MLV reverse transcriptase Promega Cat# M1701 NEBNext High-Fidelity 2x PCR Master Mix New England Biolabs Cat# M0541S SYBR green ThermoFisher Scientific Cat# S7563 Vectashield mounting medium Vector laboratories Cat# H-1000; RRID: AB_2336789 Stellaris RNA FISH Hybridization Buffer Bioresearch Technologies Cat# SMF-HB1-10 Stellaris RNA FISH Wash Buffer A Bioresearch Technologies Cat# SMF-WA1-60 Stellaris RNA FISH Wash Buffer B Bioresearch Technologies Cat# SMF-WB1-20 Critical Commercial Assays LightCycler 480 SYBR Green I Master PCR kit Roche Cat# 04707516001 Experimental Models: Cell Lines D. melanogaster Kc167 cells Drosophila Genomics Resource Center Cat# 1; RRID: CVCL_Z834 D. melanogaster S2-Dl cells Drosophila Genomics Resource Center Cat# 152; RRID: CVCL_Z993 D. melanogaster mb-MS2 cells This paper N/A Experimental Models: Organisms/Strains D. melanogaster EGFR-psqRNAi [4] N/A D. melanogaster 15B03-lexA [4] N/A D. melanogaster LexAop-MadRNAi [4] N/A D. melanogaster Ap-Gal4 Bloomington Drosophila Stock Center Cat# 25685; RRID: BDSC_25685 D. melanogaster UAS-mCD8-GFP Bloomington Drosophila Stock Center Cat# 5137; RRID: BDSC_5137 D. melanogaster Notch[NRE]-GFP [21] N/A D. melanogaster m6-GFP [22] N/A D. melanogaster UAS-Delta30B [40] N/A D. melanogaster Enh3-GFP [9] N/A D. melanogaster Enh3[mut]-GFP [9] N/A D. melanogaster Him-GFP [41] N/A D. melanogaster lexAop-CD8-GFP Bloomington Drosophila Stock Center Cat# 66545; RRID: BDSC_66545 D. melanogaster UAS-DlRNAi Bloomington Drosophila Stock Center Cat# 28032; RRID: BDSC_28032 D. melanogaster UAS-DiaRNAi Bloomington Drosophila Stock Center Cat# 33424; RRID: BDSC_33424

Article Title: Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways.
Article Snippet: doi:10.1111/febs.17288 The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways.. Autophagy-related 2 (Atg2) protein is involved in autophagosome formation through its lipid transfer function; however, other functions in animal development, especially the role of Atg2 in maintaining hematopoietic homeostasis, are unclear.. Here, we show that Atg2 knockdown in the cortical zone (CZ) induced the proliferation and differentiation of mature plasmatocytes and disrupted progenitor maintenance in the medullary zone (MZ).

Article Title: Non‐apoptotic caspase activation ensures the homeostasis of ovarian somatic stem cells
Article Snippet: Primary antibodies used in our experiments were as follows anti-Castor (1:2,000; a gift from Alex Gould); rabbit anti-HA (1:1,000; Cell Signaling C29F4); mouse anti-b-Gal (1:500; Promega Z378B); chicken Anti-b-Gal (1:200, Abcam AB9361); Anti-FasIII (1:75, Hybridoma Bank 7G10); Anti-Ci-155-full length (1:50, Hybridoma Bank 2A1); AntiPtc (1:50, Hybridoma Bank Apa1); Anti-Ref2P (1:300, Abcam 178440); Anti-mCherry (1:50, invitrogen Cat #PA5-34974).

Article Title: Draper-mediated JNK signaling is required for glial phagocytosis of apoptotic neurons during Drosophila metamorphosis.
Article Snippet: Funding information Israel Science Foundation, Grant/Award Number: 1872/15 Abstract Development of the central nervous system involves elimination of superfluous neurons through apoptosis and subsequent phagocytosis.. In Drosophila, this occurs mainly during three developmental stages: embryogenesis, metamorphosis and emerging adult.. Two transmembrane glial phagocytic receptors, SIMU (homolog of the mammalian Stabilin-2) and Draper (homolog of the mammalian MEGF10 and Jedi), mediate glial phagocytosis of apoptotic neurons during embryogenesis.

Article Title: High sugar diet disrupts gut homeostasis though JNK and STAT pathways in Drosophila.
Article Snippet: The incidence of diseases associated with a high sugar diet has increased in the past years, and numerous studies have focused on the effect of high sugar intake on obesity and metabolic syndrome.. However, how a high sugar diet influences gut homeostasis is still poorly understood.. In this study, we used Drosophila melanogaster as a model organism and supplemented a culture medium with 1 M sucrose to create a high sugar condition.

Reverse Transcription:

Article Title: Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila.
Article Snippet: Melanotic nodules are commonly assumed to be caused by an abnormal immune response.. Several hematopoietic mutants and signaling pathways, including the Toll, JAK/STAT, Ras and JNK pathways, can cause melanotic nodules to develop when specifically activated in hemocytes.. Here, we used the UASGal4 system to overexpress jumeaux (jumu) in the fly immune response system.

Article Title: Inverse regulation of target genes at the brink of the BMP morphogen activity gradient.
Article Snippet: BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates.. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk).. This patterning model is inadequate to explain the expression of target genes, like vestigial and spalt, in lateral regions of the wing disc where BMP signals decline and Brk levels peak.

Article Title: APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Article Snippet: Mouse anti-B-gal , Promega , RRID: AB_2314509.

Article Title: Notch Mediates Inter-tissue Communication to Promote Tumorigenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-GFP Abcam Cat# ab6673; RRID: AB_305643 Mouse anti-Cut DSHB Cat# 2b10; RRID: AB_528186 Rat anti-DE-Cad2 DSHB Cat# DCAD2; RRID: AB_528120 Mouse anti-Delta DHSB Cat# c594.9b; RRID: AB_528194 Mouse anti-b-Gal Promega Cat# Z378A; RRID: AB_2313752 Rat anti-Tropomyosin Abcam Cat# ab50567; RRID: AB_883223 Rabbit anti-Zfh1 Ruth Lehmann N/A Rabbit anti-Mef2 Eileen Furlong N/A Rabbit anti-Twist Eileen Furlong N/A Mouse anti-pH3 Cell Signaling Technology Cat# 9706; RRID: AB_331748 Mouse Anti-NICD DSHB Cat# c17.9c6; RRID: AB_528410 Chemicals, Peptides, and Recombinant Proteins TRIzol ThermoFisher Scientific Cat# 15596026 Oligo(dT)15 primers Promega Cat# C1101 M-MLV reverse transcriptase Promega Cat# M1701 NEBNext High-Fidelity 2x PCR Master Mix New England Biolabs Cat# M0541S SYBR green ThermoFisher Scientific Cat# S7563 Vectashield mounting medium Vector laboratories Cat# H-1000; RRID: AB_2336789 Stellaris RNA FISH Hybridization Buffer Bioresearch Technologies Cat# SMF-HB1-10 Stellaris RNA FISH Wash Buffer A Bioresearch Technologies Cat# SMF-WA1-60 Stellaris RNA FISH Wash Buffer B Bioresearch Technologies Cat# SMF-WB1-20 Critical Commercial Assays LightCycler 480 SYBR Green I Master PCR kit Roche Cat# 04707516001 Experimental Models: Cell Lines D. melanogaster Kc167 cells Drosophila Genomics Resource Center Cat# 1; RRID: CVCL_Z834 D. melanogaster S2-Dl cells Drosophila Genomics Resource Center Cat# 152; RRID: CVCL_Z993 D. melanogaster mb-MS2 cells This paper N/A Experimental Models: Organisms/Strains D. melanogaster EGFR-psqRNAi [4] N/A D. melanogaster 15B03-lexA [4] N/A D. melanogaster LexAop-MadRNAi [4] N/A D. melanogaster Ap-Gal4 Bloomington Drosophila Stock Center Cat# 25685; RRID: BDSC_25685 D. melanogaster UAS-mCD8-GFP Bloomington Drosophila Stock Center Cat# 5137; RRID: BDSC_5137 D. melanogaster Notch[NRE]-GFP [21] N/A D. melanogaster m6-GFP [22] N/A D. melanogaster UAS-Delta30B [40] N/A D. melanogaster Enh3-GFP [9] N/A D. melanogaster Enh3[mut]-GFP [9] N/A D. melanogaster Him-GFP [41] N/A D. melanogaster lexAop-CD8-GFP Bloomington Drosophila Stock Center Cat# 66545; RRID: BDSC_66545 D. melanogaster UAS-DlRNAi Bloomington Drosophila Stock Center Cat# 28032; RRID: BDSC_28032 D. melanogaster UAS-DiaRNAi Bloomington Drosophila Stock Center Cat# 33424; RRID: BDSC_33424

Article Title: Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways.
Article Snippet: doi:10.1111/febs.17288 The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways.. Autophagy-related 2 (Atg2) protein is involved in autophagosome formation through its lipid transfer function; however, other functions in animal development, especially the role of Atg2 in maintaining hematopoietic homeostasis, are unclear.. Here, we show that Atg2 knockdown in the cortical zone (CZ) induced the proliferation and differentiation of mature plasmatocytes and disrupted progenitor maintenance in the medullary zone (MZ).

Article Title: Non‐apoptotic caspase activation ensures the homeostasis of ovarian somatic stem cells
Article Snippet: Primary antibodies used in our experiments were as follows anti-Castor (1:2,000; a gift from Alex Gould); rabbit anti-HA (1:1,000; Cell Signaling C29F4); mouse anti-b-Gal (1:500; Promega Z378B); chicken Anti-b-Gal (1:200, Abcam AB9361); Anti-FasIII (1:75, Hybridoma Bank 7G10); Anti-Ci-155-full length (1:50, Hybridoma Bank 2A1); AntiPtc (1:50, Hybridoma Bank Apa1); Anti-Ref2P (1:300, Abcam 178440); Anti-mCherry (1:50, invitrogen Cat #PA5-34974).

Article Title: Draper-mediated JNK signaling is required for glial phagocytosis of apoptotic neurons during Drosophila metamorphosis.
Article Snippet: Funding information Israel Science Foundation, Grant/Award Number: 1872/15 Abstract Development of the central nervous system involves elimination of superfluous neurons through apoptosis and subsequent phagocytosis.. In Drosophila, this occurs mainly during three developmental stages: embryogenesis, metamorphosis and emerging adult.. Two transmembrane glial phagocytic receptors, SIMU (homolog of the mammalian Stabilin-2) and Draper (homolog of the mammalian MEGF10 and Jedi), mediate glial phagocytosis of apoptotic neurons during embryogenesis.

Article Title: High sugar diet disrupts gut homeostasis though JNK and STAT pathways in Drosophila.
Article Snippet: The incidence of diseases associated with a high sugar diet has increased in the past years, and numerous studies have focused on the effect of high sugar intake on obesity and metabolic syndrome.. However, how a high sugar diet influences gut homeostasis is still poorly understood.. In this study, we used Drosophila melanogaster as a model organism and supplemented a culture medium with 1 M sucrose to create a high sugar condition.

Polymerase Chain Reaction:

Article Title: Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila.
Article Snippet: Melanotic nodules are commonly assumed to be caused by an abnormal immune response.. Several hematopoietic mutants and signaling pathways, including the Toll, JAK/STAT, Ras and JNK pathways, can cause melanotic nodules to develop when specifically activated in hemocytes.. Here, we used the UASGal4 system to overexpress jumeaux (jumu) in the fly immune response system.

Article Title: Inverse regulation of target genes at the brink of the BMP morphogen activity gradient.
Article Snippet: BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates.. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk).. This patterning model is inadequate to explain the expression of target genes, like vestigial and spalt, in lateral regions of the wing disc where BMP signals decline and Brk levels peak.

Article Title: APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Article Snippet: Mouse anti-B-gal , Promega , RRID: AB_2314509.

Article Title: Notch Mediates Inter-tissue Communication to Promote Tumorigenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-GFP Abcam Cat# ab6673; RRID: AB_305643 Mouse anti-Cut DSHB Cat# 2b10; RRID: AB_528186 Rat anti-DE-Cad2 DSHB Cat# DCAD2; RRID: AB_528120 Mouse anti-Delta DHSB Cat# c594.9b; RRID: AB_528194 Mouse anti-b-Gal Promega Cat# Z378A; RRID: AB_2313752 Rat anti-Tropomyosin Abcam Cat# ab50567; RRID: AB_883223 Rabbit anti-Zfh1 Ruth Lehmann N/A Rabbit anti-Mef2 Eileen Furlong N/A Rabbit anti-Twist Eileen Furlong N/A Mouse anti-pH3 Cell Signaling Technology Cat# 9706; RRID: AB_331748 Mouse Anti-NICD DSHB Cat# c17.9c6; RRID: AB_528410 Chemicals, Peptides, and Recombinant Proteins TRIzol ThermoFisher Scientific Cat# 15596026 Oligo(dT)15 primers Promega Cat# C1101 M-MLV reverse transcriptase Promega Cat# M1701 NEBNext High-Fidelity 2x PCR Master Mix New England Biolabs Cat# M0541S SYBR green ThermoFisher Scientific Cat# S7563 Vectashield mounting medium Vector laboratories Cat# H-1000; RRID: AB_2336789 Stellaris RNA FISH Hybridization Buffer Bioresearch Technologies Cat# SMF-HB1-10 Stellaris RNA FISH Wash Buffer A Bioresearch Technologies Cat# SMF-WA1-60 Stellaris RNA FISH Wash Buffer B Bioresearch Technologies Cat# SMF-WB1-20 Critical Commercial Assays LightCycler 480 SYBR Green I Master PCR kit Roche Cat# 04707516001 Experimental Models: Cell Lines D. melanogaster Kc167 cells Drosophila Genomics Resource Center Cat# 1; RRID: CVCL_Z834 D. melanogaster S2-Dl cells Drosophila Genomics Resource Center Cat# 152; RRID: CVCL_Z993 D. melanogaster mb-MS2 cells This paper N/A Experimental Models: Organisms/Strains D. melanogaster EGFR-psqRNAi [4] N/A D. melanogaster 15B03-lexA [4] N/A D. melanogaster LexAop-MadRNAi [4] N/A D. melanogaster Ap-Gal4 Bloomington Drosophila Stock Center Cat# 25685; RRID: BDSC_25685 D. melanogaster UAS-mCD8-GFP Bloomington Drosophila Stock Center Cat# 5137; RRID: BDSC_5137 D. melanogaster Notch[NRE]-GFP [21] N/A D. melanogaster m6-GFP [22] N/A D. melanogaster UAS-Delta30B [40] N/A D. melanogaster Enh3-GFP [9] N/A D. melanogaster Enh3[mut]-GFP [9] N/A D. melanogaster Him-GFP [41] N/A D. melanogaster lexAop-CD8-GFP Bloomington Drosophila Stock Center Cat# 66545; RRID: BDSC_66545 D. melanogaster UAS-DlRNAi Bloomington Drosophila Stock Center Cat# 28032; RRID: BDSC_28032 D. melanogaster UAS-DiaRNAi Bloomington Drosophila Stock Center Cat# 33424; RRID: BDSC_33424

Article Title: Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways.
Article Snippet: doi:10.1111/febs.17288 The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways.. Autophagy-related 2 (Atg2) protein is involved in autophagosome formation through its lipid transfer function; however, other functions in animal development, especially the role of Atg2 in maintaining hematopoietic homeostasis, are unclear.. Here, we show that Atg2 knockdown in the cortical zone (CZ) induced the proliferation and differentiation of mature plasmatocytes and disrupted progenitor maintenance in the medullary zone (MZ).

Article Title: Non‐apoptotic caspase activation ensures the homeostasis of ovarian somatic stem cells
Article Snippet: Primary antibodies used in our experiments were as follows anti-Castor (1:2,000; a gift from Alex Gould); rabbit anti-HA (1:1,000; Cell Signaling C29F4); mouse anti-b-Gal (1:500; Promega Z378B); chicken Anti-b-Gal (1:200, Abcam AB9361); Anti-FasIII (1:75, Hybridoma Bank 7G10); Anti-Ci-155-full length (1:50, Hybridoma Bank 2A1); AntiPtc (1:50, Hybridoma Bank Apa1); Anti-Ref2P (1:300, Abcam 178440); Anti-mCherry (1:50, invitrogen Cat #PA5-34974).

Article Title: Draper-mediated JNK signaling is required for glial phagocytosis of apoptotic neurons during Drosophila metamorphosis.
Article Snippet: Funding information Israel Science Foundation, Grant/Award Number: 1872/15 Abstract Development of the central nervous system involves elimination of superfluous neurons through apoptosis and subsequent phagocytosis.. In Drosophila, this occurs mainly during three developmental stages: embryogenesis, metamorphosis and emerging adult.. Two transmembrane glial phagocytic receptors, SIMU (homolog of the mammalian Stabilin-2) and Draper (homolog of the mammalian MEGF10 and Jedi), mediate glial phagocytosis of apoptotic neurons during embryogenesis.

Article Title: High sugar diet disrupts gut homeostasis though JNK and STAT pathways in Drosophila.
Article Snippet: The incidence of diseases associated with a high sugar diet has increased in the past years, and numerous studies have focused on the effect of high sugar intake on obesity and metabolic syndrome.. However, how a high sugar diet influences gut homeostasis is still poorly understood.. In this study, we used Drosophila melanogaster as a model organism and supplemented a culture medium with 1 M sucrose to create a high sugar condition.

SYBR Green Assay:

Article Title: Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila.
Article Snippet: Melanotic nodules are commonly assumed to be caused by an abnormal immune response.. Several hematopoietic mutants and signaling pathways, including the Toll, JAK/STAT, Ras and JNK pathways, can cause melanotic nodules to develop when specifically activated in hemocytes.. Here, we used the UASGal4 system to overexpress jumeaux (jumu) in the fly immune response system.

Article Title: Inverse regulation of target genes at the brink of the BMP morphogen activity gradient.
Article Snippet: BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates.. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk).. This patterning model is inadequate to explain the expression of target genes, like vestigial and spalt, in lateral regions of the wing disc where BMP signals decline and Brk levels peak.

Article Title: APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Article Snippet: Mouse anti-B-gal , Promega , RRID: AB_2314509.

Article Title: Notch Mediates Inter-tissue Communication to Promote Tumorigenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-GFP Abcam Cat# ab6673; RRID: AB_305643 Mouse anti-Cut DSHB Cat# 2b10; RRID: AB_528186 Rat anti-DE-Cad2 DSHB Cat# DCAD2; RRID: AB_528120 Mouse anti-Delta DHSB Cat# c594.9b; RRID: AB_528194 Mouse anti-b-Gal Promega Cat# Z378A; RRID: AB_2313752 Rat anti-Tropomyosin Abcam Cat# ab50567; RRID: AB_883223 Rabbit anti-Zfh1 Ruth Lehmann N/A Rabbit anti-Mef2 Eileen Furlong N/A Rabbit anti-Twist Eileen Furlong N/A Mouse anti-pH3 Cell Signaling Technology Cat# 9706; RRID: AB_331748 Mouse Anti-NICD DSHB Cat# c17.9c6; RRID: AB_528410 Chemicals, Peptides, and Recombinant Proteins TRIzol ThermoFisher Scientific Cat# 15596026 Oligo(dT)15 primers Promega Cat# C1101 M-MLV reverse transcriptase Promega Cat# M1701 NEBNext High-Fidelity 2x PCR Master Mix New England Biolabs Cat# M0541S SYBR green ThermoFisher Scientific Cat# S7563 Vectashield mounting medium Vector laboratories Cat# H-1000; RRID: AB_2336789 Stellaris RNA FISH Hybridization Buffer Bioresearch Technologies Cat# SMF-HB1-10 Stellaris RNA FISH Wash Buffer A Bioresearch Technologies Cat# SMF-WA1-60 Stellaris RNA FISH Wash Buffer B Bioresearch Technologies Cat# SMF-WB1-20 Critical Commercial Assays LightCycler 480 SYBR Green I Master PCR kit Roche Cat# 04707516001 Experimental Models: Cell Lines D. melanogaster Kc167 cells Drosophila Genomics Resource Center Cat# 1; RRID: CVCL_Z834 D. melanogaster S2-Dl cells Drosophila Genomics Resource Center Cat# 152; RRID: CVCL_Z993 D. melanogaster mb-MS2 cells This paper N/A Experimental Models: Organisms/Strains D. melanogaster EGFR-psqRNAi [4] N/A D. melanogaster 15B03-lexA [4] N/A D. melanogaster LexAop-MadRNAi [4] N/A D. melanogaster Ap-Gal4 Bloomington Drosophila Stock Center Cat# 25685; RRID: BDSC_25685 D. melanogaster UAS-mCD8-GFP Bloomington Drosophila Stock Center Cat# 5137; RRID: BDSC_5137 D. melanogaster Notch[NRE]-GFP [21] N/A D. melanogaster m6-GFP [22] N/A D. melanogaster UAS-Delta30B [40] N/A D. melanogaster Enh3-GFP [9] N/A D. melanogaster Enh3[mut]-GFP [9] N/A D. melanogaster Him-GFP [41] N/A D. melanogaster lexAop-CD8-GFP Bloomington Drosophila Stock Center Cat# 66545; RRID: BDSC_66545 D. melanogaster UAS-DlRNAi Bloomington Drosophila Stock Center Cat# 28032; RRID: BDSC_28032 D. melanogaster UAS-DiaRNAi Bloomington Drosophila Stock Center Cat# 33424; RRID: BDSC_33424

Article Title: Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways.
Article Snippet: doi:10.1111/febs.17288 The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways.. Autophagy-related 2 (Atg2) protein is involved in autophagosome formation through its lipid transfer function; however, other functions in animal development, especially the role of Atg2 in maintaining hematopoietic homeostasis, are unclear.. Here, we show that Atg2 knockdown in the cortical zone (CZ) induced the proliferation and differentiation of mature plasmatocytes and disrupted progenitor maintenance in the medullary zone (MZ).

Article Title: Non‐apoptotic caspase activation ensures the homeostasis of ovarian somatic stem cells
Article Snippet: Primary antibodies used in our experiments were as follows anti-Castor (1:2,000; a gift from Alex Gould); rabbit anti-HA (1:1,000; Cell Signaling C29F4); mouse anti-b-Gal (1:500; Promega Z378B); chicken Anti-b-Gal (1:200, Abcam AB9361); Anti-FasIII (1:75, Hybridoma Bank 7G10); Anti-Ci-155-full length (1:50, Hybridoma Bank 2A1); AntiPtc (1:50, Hybridoma Bank Apa1); Anti-Ref2P (1:300, Abcam 178440); Anti-mCherry (1:50, invitrogen Cat #PA5-34974).

Article Title: Draper-mediated JNK signaling is required for glial phagocytosis of apoptotic neurons during Drosophila metamorphosis.
Article Snippet: Funding information Israel Science Foundation, Grant/Award Number: 1872/15 Abstract Development of the central nervous system involves elimination of superfluous neurons through apoptosis and subsequent phagocytosis.. In Drosophila, this occurs mainly during three developmental stages: embryogenesis, metamorphosis and emerging adult.. Two transmembrane glial phagocytic receptors, SIMU (homolog of the mammalian Stabilin-2) and Draper (homolog of the mammalian MEGF10 and Jedi), mediate glial phagocytosis of apoptotic neurons during embryogenesis.

Article Title: High sugar diet disrupts gut homeostasis though JNK and STAT pathways in Drosophila.
Article Snippet: The incidence of diseases associated with a high sugar diet has increased in the past years, and numerous studies have focused on the effect of high sugar intake on obesity and metabolic syndrome.. However, how a high sugar diet influences gut homeostasis is still poorly understood.. In this study, we used Drosophila melanogaster as a model organism and supplemented a culture medium with 1 M sucrose to create a high sugar condition.

Fluorescence In Situ Hybridization:

Article Title: Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila.
Article Snippet: Melanotic nodules are commonly assumed to be caused by an abnormal immune response.. Several hematopoietic mutants and signaling pathways, including the Toll, JAK/STAT, Ras and JNK pathways, can cause melanotic nodules to develop when specifically activated in hemocytes.. Here, we used the UASGal4 system to overexpress jumeaux (jumu) in the fly immune response system.

Article Title: Inverse regulation of target genes at the brink of the BMP morphogen activity gradient.
Article Snippet: BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates.. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk).. This patterning model is inadequate to explain the expression of target genes, like vestigial and spalt, in lateral regions of the wing disc where BMP signals decline and Brk levels peak.

Article Title: APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Article Snippet: Mouse anti-B-gal , Promega , RRID: AB_2314509.

Article Title: Notch Mediates Inter-tissue Communication to Promote Tumorigenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-GFP Abcam Cat# ab6673; RRID: AB_305643 Mouse anti-Cut DSHB Cat# 2b10; RRID: AB_528186 Rat anti-DE-Cad2 DSHB Cat# DCAD2; RRID: AB_528120 Mouse anti-Delta DHSB Cat# c594.9b; RRID: AB_528194 Mouse anti-b-Gal Promega Cat# Z378A; RRID: AB_2313752 Rat anti-Tropomyosin Abcam Cat# ab50567; RRID: AB_883223 Rabbit anti-Zfh1 Ruth Lehmann N/A Rabbit anti-Mef2 Eileen Furlong N/A Rabbit anti-Twist Eileen Furlong N/A Mouse anti-pH3 Cell Signaling Technology Cat# 9706; RRID: AB_331748 Mouse Anti-NICD DSHB Cat# c17.9c6; RRID: AB_528410 Chemicals, Peptides, and Recombinant Proteins TRIzol ThermoFisher Scientific Cat# 15596026 Oligo(dT)15 primers Promega Cat# C1101 M-MLV reverse transcriptase Promega Cat# M1701 NEBNext High-Fidelity 2x PCR Master Mix New England Biolabs Cat# M0541S SYBR green ThermoFisher Scientific Cat# S7563 Vectashield mounting medium Vector laboratories Cat# H-1000; RRID: AB_2336789 Stellaris RNA FISH Hybridization Buffer Bioresearch Technologies Cat# SMF-HB1-10 Stellaris RNA FISH Wash Buffer A Bioresearch Technologies Cat# SMF-WA1-60 Stellaris RNA FISH Wash Buffer B Bioresearch Technologies Cat# SMF-WB1-20 Critical Commercial Assays LightCycler 480 SYBR Green I Master PCR kit Roche Cat# 04707516001 Experimental Models: Cell Lines D. melanogaster Kc167 cells Drosophila Genomics Resource Center Cat# 1; RRID: CVCL_Z834 D. melanogaster S2-Dl cells Drosophila Genomics Resource Center Cat# 152; RRID: CVCL_Z993 D. melanogaster mb-MS2 cells This paper N/A Experimental Models: Organisms/Strains D. melanogaster EGFR-psqRNAi [4] N/A D. melanogaster 15B03-lexA [4] N/A D. melanogaster LexAop-MadRNAi [4] N/A D. melanogaster Ap-Gal4 Bloomington Drosophila Stock Center Cat# 25685; RRID: BDSC_25685 D. melanogaster UAS-mCD8-GFP Bloomington Drosophila Stock Center Cat# 5137; RRID: BDSC_5137 D. melanogaster Notch[NRE]-GFP [21] N/A D. melanogaster m6-GFP [22] N/A D. melanogaster UAS-Delta30B [40] N/A D. melanogaster Enh3-GFP [9] N/A D. melanogaster Enh3[mut]-GFP [9] N/A D. melanogaster Him-GFP [41] N/A D. melanogaster lexAop-CD8-GFP Bloomington Drosophila Stock Center Cat# 66545; RRID: BDSC_66545 D. melanogaster UAS-DlRNAi Bloomington Drosophila Stock Center Cat# 28032; RRID: BDSC_28032 D. melanogaster UAS-DiaRNAi Bloomington Drosophila Stock Center Cat# 33424; RRID: BDSC_33424

Article Title: Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways.
Article Snippet: doi:10.1111/febs.17288 The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways.. Autophagy-related 2 (Atg2) protein is involved in autophagosome formation through its lipid transfer function; however, other functions in animal development, especially the role of Atg2 in maintaining hematopoietic homeostasis, are unclear.. Here, we show that Atg2 knockdown in the cortical zone (CZ) induced the proliferation and differentiation of mature plasmatocytes and disrupted progenitor maintenance in the medullary zone (MZ).

Article Title: Non‐apoptotic caspase activation ensures the homeostasis of ovarian somatic stem cells
Article Snippet: Primary antibodies used in our experiments were as follows anti-Castor (1:2,000; a gift from Alex Gould); rabbit anti-HA (1:1,000; Cell Signaling C29F4); mouse anti-b-Gal (1:500; Promega Z378B); chicken Anti-b-Gal (1:200, Abcam AB9361); Anti-FasIII (1:75, Hybridoma Bank 7G10); Anti-Ci-155-full length (1:50, Hybridoma Bank 2A1); AntiPtc (1:50, Hybridoma Bank Apa1); Anti-Ref2P (1:300, Abcam 178440); Anti-mCherry (1:50, invitrogen Cat #PA5-34974).

Article Title: Draper-mediated JNK signaling is required for glial phagocytosis of apoptotic neurons during Drosophila metamorphosis.
Article Snippet: Funding information Israel Science Foundation, Grant/Award Number: 1872/15 Abstract Development of the central nervous system involves elimination of superfluous neurons through apoptosis and subsequent phagocytosis.. In Drosophila, this occurs mainly during three developmental stages: embryogenesis, metamorphosis and emerging adult.. Two transmembrane glial phagocytic receptors, SIMU (homolog of the mammalian Stabilin-2) and Draper (homolog of the mammalian MEGF10 and Jedi), mediate glial phagocytosis of apoptotic neurons during embryogenesis.

Article Title: High sugar diet disrupts gut homeostasis though JNK and STAT pathways in Drosophila.
Article Snippet: The incidence of diseases associated with a high sugar diet has increased in the past years, and numerous studies have focused on the effect of high sugar intake on obesity and metabolic syndrome.. However, how a high sugar diet influences gut homeostasis is still poorly understood.. In this study, we used Drosophila melanogaster as a model organism and supplemented a culture medium with 1 M sucrose to create a high sugar condition.

Hybridization:

Article Title: Overexpression of jumu induces melanotic nodules by activating Toll signaling in Drosophila.
Article Snippet: Melanotic nodules are commonly assumed to be caused by an abnormal immune response.. Several hematopoietic mutants and signaling pathways, including the Toll, JAK/STAT, Ras and JNK pathways, can cause melanotic nodules to develop when specifically activated in hemocytes.. Here, we used the UASGal4 system to overexpress jumeaux (jumu) in the fly immune response system.

Article Title: Inverse regulation of target genes at the brink of the BMP morphogen activity gradient.
Article Snippet: BMP-dependent patterning in the Drosophila melanogaster wing imaginal disc serves as a paradigm to understand how morphogens specify cell fates.. The observed profile of the transcriptional response to the graded signal of BMP relies upon two counter-active gradients of pMad and Brinker (Brk).. This patterning model is inadequate to explain the expression of target genes, like vestigial and spalt, in lateral regions of the wing disc where BMP signals decline and Brk levels peak.

Article Title: APC Inhibits Ligand-Independent Wnt Signaling by the Clathrin Endocytic Pathway
Article Snippet: Mouse anti-B-gal , Promega , RRID: AB_2314509.

Article Title: Notch Mediates Inter-tissue Communication to Promote Tumorigenesis.
Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Goat anti-GFP Abcam Cat# ab6673; RRID: AB_305643 Mouse anti-Cut DSHB Cat# 2b10; RRID: AB_528186 Rat anti-DE-Cad2 DSHB Cat# DCAD2; RRID: AB_528120 Mouse anti-Delta DHSB Cat# c594.9b; RRID: AB_528194 Mouse anti-b-Gal Promega Cat# Z378A; RRID: AB_2313752 Rat anti-Tropomyosin Abcam Cat# ab50567; RRID: AB_883223 Rabbit anti-Zfh1 Ruth Lehmann N/A Rabbit anti-Mef2 Eileen Furlong N/A Rabbit anti-Twist Eileen Furlong N/A Mouse anti-pH3 Cell Signaling Technology Cat# 9706; RRID: AB_331748 Mouse Anti-NICD DSHB Cat# c17.9c6; RRID: AB_528410 Chemicals, Peptides, and Recombinant Proteins TRIzol ThermoFisher Scientific Cat# 15596026 Oligo(dT)15 primers Promega Cat# C1101 M-MLV reverse transcriptase Promega Cat# M1701 NEBNext High-Fidelity 2x PCR Master Mix New England Biolabs Cat# M0541S SYBR green ThermoFisher Scientific Cat# S7563 Vectashield mounting medium Vector laboratories Cat# H-1000; RRID: AB_2336789 Stellaris RNA FISH Hybridization Buffer Bioresearch Technologies Cat# SMF-HB1-10 Stellaris RNA FISH Wash Buffer A Bioresearch Technologies Cat# SMF-WA1-60 Stellaris RNA FISH Wash Buffer B Bioresearch Technologies Cat# SMF-WB1-20 Critical Commercial Assays LightCycler 480 SYBR Green I Master PCR kit Roche Cat# 04707516001 Experimental Models: Cell Lines D. melanogaster Kc167 cells Drosophila Genomics Resource Center Cat# 1; RRID: CVCL_Z834 D. melanogaster S2-Dl cells Drosophila Genomics Resource Center Cat# 152; RRID: CVCL_Z993 D. melanogaster mb-MS2 cells This paper N/A Experimental Models: Organisms/Strains D. melanogaster EGFR-psqRNAi [4] N/A D. melanogaster 15B03-lexA [4] N/A D. melanogaster LexAop-MadRNAi [4] N/A D. melanogaster Ap-Gal4 Bloomington Drosophila Stock Center Cat# 25685; RRID: BDSC_25685 D. melanogaster UAS-mCD8-GFP Bloomington Drosophila Stock Center Cat# 5137; RRID: BDSC_5137 D. melanogaster Notch[NRE]-GFP [21] N/A D. melanogaster m6-GFP [22] N/A D. melanogaster UAS-Delta30B [40] N/A D. melanogaster Enh3-GFP [9] N/A D. melanogaster Enh3[mut]-GFP [9] N/A D. melanogaster Him-GFP [41] N/A D. melanogaster lexAop-CD8-GFP Bloomington Drosophila Stock Center Cat# 66545; RRID: BDSC_66545 D. melanogaster UAS-DlRNAi Bloomington Drosophila Stock Center Cat# 28032; RRID: BDSC_28032 D. melanogaster UAS-DiaRNAi Bloomington Drosophila Stock Center Cat# 33424; RRID: BDSC_33424

Article Title: Atg2 controls Drosophila hematopoiesis through the PVR/TOR signaling pathways.
Article Snippet: doi:10.1111/febs.17288 The hematopoietic system of Drosophila is a well-established genetic model for studying hematopoiesis mechanisms, which are strictly regulated by multiple signaling pathways.. Autophagy-related 2 (Atg2) protein is involved in autophagosome formation through its lipid transfer function; however, other functions in animal development, especially the role of Atg2 in maintaining hematopoietic homeostasis, are unclear.. Here, we show that Atg2 knockdown in the cortical zone (CZ) induced the proliferation and differentiation of mature plasmatocytes and disrupted progenitor maintenance in the medullary zone (MZ).

Article Title: Non‐apoptotic caspase activation ensures the homeostasis of ovarian somatic stem cells
Article Snippet: Primary antibodies used in our experiments were as follows anti-Castor (1:2,000; a gift from Alex Gould); rabbit anti-HA (1:1,000; Cell Signaling C29F4); mouse anti-b-Gal (1:500; Promega Z378B); chicken Anti-b-Gal (1:200, Abcam AB9361); Anti-FasIII (1:75, Hybridoma Bank 7G10); Anti-Ci-155-full length (1:50, Hybridoma Bank 2A1); AntiPtc (1:50, Hybridoma Bank Apa1); Anti-Ref2P (1:300, Abcam 178440); Anti-mCherry (1:50, invitrogen Cat #PA5-34974).

Article Title: Draper-mediated JNK signaling is required for glial phagocytosis of apoptotic neurons during Drosophila metamorphosis.
Article Snippet: Funding information Israel Science Foundation, Grant/Award Number: 1872/15 Abstract Development of the central nervous system involves elimination of superfluous neurons through apoptosis and subsequent phagocytosis.. In Drosophila, this occurs mainly during three developmental stages: embryogenesis, metamorphosis and emerging adult.. Two transmembrane glial phagocytic receptors, SIMU (homolog of the mammalian Stabilin-2) and Draper (homolog of the mammalian MEGF10 and Jedi), mediate glial phagocytosis of apoptotic neurons during embryogenesis.

Article Title: High sugar diet disrupts gut homeostasis though JNK and STAT pathways in Drosophila.
Article Snippet: The incidence of diseases associated with a high sugar diet has increased in the past years, and numerous studies have focused on the effect of high sugar intake on obesity and metabolic syndrome.. However, how a high sugar diet influences gut homeostasis is still poorly understood.. In this study, we used Drosophila melanogaster as a model organism and supplemented a culture medium with 1 M sucrose to create a high sugar condition.



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